I believe this is a Datura, also called the Devil’s Trumpet, it blooms at night and wilts in the day (if this is wrong, tell me). Used a flash with a diffusion card over the front and sidelit it as to not have light spill into the background. Also used the 1/250th sync to get rid of any ambient light. The hard part was the focusing, which consisted of setting the lens to it’s minimum focusing distance and moving either closer or further away from it while snapping photos until I had one in focus. Oh yeah, this is not studio.

Canon 30D
ISO: 400
F/7.1 @ 1/250
Lit with SB-28
24mm FL